Clone: F12B.
Background: Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas.
Positive Control: Rhabdomyosarcoma and Wlim's tumors.
Purification Method: Protein A/G Chromatography.
Source: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1. Wright WE, et al. Monoclonal antimyogenin antibodies define epitopes outside the bHLH domain where binding interferes with protein-protein and protein-DNA interaction. Dev Genet 19: 131-138 (1996).
2. Wright WE, et al. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD. Cell 1989; 56:607.
3. Aurade F, et al. Myf5, MyoD, myogenin and MRF4 myogenic derivatives of the embryonic mesenchymal cell line C3H10T1/2 exhibit the same adult muscle phenotype. Differentiation 1994; 55:185.
UniProt: P15173 (Human)
P12979 (Mouse)
P20428 (Rat).
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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