Clone: 16E4.
Background: The discovery of NPDC-1 (neuronal proliferation, differentiation and control protein 1) was the result of a study of genes that were preferentially expressed in immortalized neural precursor cell lines at the onset of contact inhibition of proliferation and subsequent differentiation. It was further found that transfection of transformed cells with NPDC-1 led to increased doubling times and suppression of many characteristics of transformation. NPDC-1 is differentially expressed in brain and lung and has been shown to interact directly with the transcription factor E2F-1 as well as D-type cyclins and cdk2. NPDC-1 has been identified as a modulator of transcriptiona levents mediated by retinoic acid and the protein level and activity of NPDC-1 have been shown to be regulated by phosphorylation mediated proteasomal degradation.
Positive Control: Brain tissue lysate (mouse and rat tested, human not yet tested).
Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with a synthetic peptide derived from human NPDC-1 protein and mouse myeloma cells.
Formulation: 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, pH 7.5, 3mM sodium azide.
References: 1. Spencer, M.L., et al. 'NPDC-1, a novel regulator of neuronal proliferation, is degraded by the ubiquitin/proteasome system through a PEST degradation motif.' J. Biol. Chem. (2004), 279, 37069-37078
2. Evrard, C. & Rouget P. 'Subcellular localization of neural-specific NPDC-1 protein.' J Neurosci Res (2005), 79, 747-755
3. Sansal, I., et al. 'NPDC-1, a regulator of neural cell proliferation and differentiation, interacts with E2F-1, reduces its binding to DNA and modulates its transcriptional activity.' Oncogene (2000), 19, 5000-5009.
UniProt: Q8WXX4.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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