Clone: C2M
Background: Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromosome proteins are conserved in eukaryotes lead to mitotic chromosome segregation defects, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. Specifically this SMC1/SMC3 complex is responsible for sister chromatid cohesion during metaphase. A number of cellular factors interact with hSMC1/hSMC3 during cell cycle. The major population of hSMC1/hSMC3 is in a compex with hRAD21 forming the human cohesion complex. Human cohesion associates with chromosomes which peaks at S phase and dissociates from chromosomes during G2/M transition. In addition, a subpopulation of hSMC1/hSMC3 associates tightly with nuclear matrix and centrosomes during interphase. A subset of hSMC1/hSMC3 is localized to spindle poles, spindles and kinetochores during mitosis when cohesin is in the cytoplasm. hSMC1/hSMC3 is required for spindle aster formation in vitro and reacts with nuclear mitotic apparatus (2) protein in vivo.
Positive Control: HeLa cells
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Source: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant protein corresponding to amino acids 402-894 of human SMC1 and mouse myeloma cells.
References: 1. Ejjpe, M., et al. 'Association of mammalian SMC1 and SMC3 proteins with meiotic chromosomes and synaptonemal complexes.' J. Cell Sci. 2000, 113, 673-682.2. Stursberg, S., et al. 'Cloning and characterization of mammalian SMC1 and SMC3 genes and proteins, components of the DNA recombination complexes RC-1.' Gene 1999, 228, 1-12.3. Strunnikov, A.V., et al. 'SMC1: an essential yeast gene encoding a putative head-rod-tail protein is required for nuclear division and defines a new ubiquitous protein family.' J. Cell Biol. 1993, 123, 1635-1648.
UniProt: Q14683
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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