Clone Number: Ks 9.70/Ks 9.216
Immunogen: Synthetic peptides aa 450-477 and aa 4-28 of human keratin K9
Concentration: n/a
Format: Supernatant
Storage buffer: Hybridoma cell culture supernatant containing 0.09% sodium azide
Additional info: For IHC-P protease treatment and/or microwave treatment recommended). The antibody reagent represents an excellent marker to study palmoplantar epidermal distribution and differentiation. Specifically reactive in the middle/upper suprabasal layers (stratum spinosum/ granulosum) of the epidermis of palm and sole. K9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). K9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. No labelling has been found in epithelial cells of other stratified epithelia such as oesophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum. The antibody cocktail is an excellent tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment.
References: Thul PJ et al. Science 2017; 356(6340):eaal3321
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