Clone Number: LAT-01
Immunogen: Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT.
Concentration: 1 mg/ml
Storage buffer: Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Additional info: LAT (linker for activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation. Immunoprecipitation: Tyrosine-phosphorylated form of LAT is not optimally recognized. Flow cytometry: Recommended dilution: 1-4 µg/ml, intracellular staining. Tyrosine-phosphorylated form of LAT is not optimally recognized.Western blotting: Recommended dilution: 1-2 μg/ml.
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