Clone Number: EM-06
Immunogen: Purified recombinant second SH3 domain of human Nck1 fused to GST
Concentration: 1 mg/ml
Format: Purified by protein-A affinity chromatography.
Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Additional info: NCK1 (NCK alpha) is a cytoplasmic adaptor protein that plays a universal role in coordinating the signaling networks critical for organizing the actin cytoskeleton, cell movement, or axon guidance, connecting transmembrane receptors to multiple intracellular signaling pathways. It contains one SH2 domain, through which NCK1 binds to phosphorylated domains of transmembrane signaling moleculs or certain adaptor proteins, and three SH3 domains for binding proline-rich sequences of other molecules involved in the process of nucleation and polymerization of the actin cytoskeleton.SpecificityThe antibody NF-05 recognizes a nonphosphorylated epitope of neurofilament heavy protein (NF-H), a 210 kDa intracellular structural protein of Intermediate Filament Proteins family. NF-H is mainly expressed in the central and peripheral nervous system and reproductive system and is biochemically very stable.Application detailsWestern blotting: Recommended dilution: 2 μg/ml; positive control: Jurkat cell lysate.
Immunocytochemistry: Recommended dilution: 2 μg/ml; positive control: human NCK1-transfected COS-7 cells.
English
Deutsch
