Clone Number: PCLO-01
Immunogen: Human recombinant PCLO protein
Concentration: 1 mg/ml
Format: Purified by protein-A affinity chromatography.
Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Additional info: PCLO (piccolo, also known as aczonin) is a large (more than 400 kDa) multidomain protein of the presynaptic cytomatrix in neurons, that is present in all vertebrate synapses, but is absent from invertebrates. It contains zinc finger and coiled-coil sequences, as well as N-terminal glutamine-rich sequence, and C-terminal PDZ domain followed by two C2 domains (C2A and C2B). In vitro binding and transfection experiments suggested that PCLO binds to multiple proteins including profilin and L-type calcium channels. It is involved in neurotransmitter release and insulin secretion.SpecificityThe mouse monoclonal antibody PC10 (also known as 3F81) recognizes PCNA, a 36 kDa conserved nuclear protein serving as a cofactor for DNA synthesis.Application detailsFlow cytometry: Recommended dilution: 1-5 μg/ml, intracellular staining.
Western blotting: A small splice variant of PCLO can be analyzed by Western blotting, however, the whole protein is too large (over 400 kDa) to be successfully processed by SDS PAAGE.
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