Mouse anti-Perforin Purified, clone dG9 (Monoclonal)

Clone Number: dG9

Immunogen: purified granules from human YT lymphoma cell line

Concentration: 1 mg/ml

Format: Purified by protein-A affinity chromatography.

Storage buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Additional info: Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.SpecificityThe mouse monoclonal antibody 7E11/C5 recognizes an epitope within the sequence FTNEDELYNLLTDSP of PPM1D, a protein phosphatase, which contributes to development of some carcinomas.Application detailsFlow cytometry: Recommended dilution: 3-12 μg/ml. Intracellular staining.

• SKU: SANMON23296
• Manufacturer: Sanbio / Monosan
• Manufacturer SKU: MON23296
• Green Labware: No
• Package Unit: 0,1 mg
• Quantity Unit: STK
• Reactivity: Human, Cow (Bovine)
• Clonality: Monoclonal
• Application: Immunohistochemistry (frozen), Immunohistochemistry (paraffin), Immunoprecipitation, Flow Cytometry, Immunocytochemistry
• Isotype: IgG2b kappa
• Host: Mouse