Clone: 5G10.
Background: The rat alpha 2b adrenergic receptor is a G protein coupled receptor which acts through Gi2 and is responsible for Cellular communication and signal transduction. These receptors are linked to pertussis toxin-sensitive G proteins. Agonist binding leads to inhibition of adenylyl cyclase, inhibition of Ca(2+) channels and activation of K(+) channels. Recently, three distinct subtypes of alpha(2)-AR were described, alpha(2A)-AR, alpha(2B)-AR and alpha(2C)-AR. At present, it is unknown which of these alpha(2)-AR subtype(s) may regulate insulin secretion.
Positive Control: MDCK cells transfected with rat alpha2B adrenergic receptors.
Purification Method: Protein A/G Chromatography.
Source: Mouse monoclonal antibody raised to a synthetic peptide corresponding to the third intracellular loop of the rat alpha 2b adrenergic receptor.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1. Ozawa, Y, et al. 'Alpha2-adrenergic receptor subtype alterations in the brainstem in the sudden infant death syndrome.' Early Hum Dev 2003, 75 Suppl, S129-38
2. Yun, J., et al. 'Gene expression profile of neurodegeneration induced by alpha1B-adrenergic receptor overactivity: NMDA/GABAA dysregulation and apoptosis.' Brain 2003, Pt 12, 2667-81
3. Peterhoff, M., et al. 'Inhibition of insulin secretion via distinct signaling pathways in alpha2-adrenoceptor knockout mice.' Eur J Endocrinol 2003, 4, 343-50
4. Paris, A., et al. 'Activation of alpha 2B-adrenoceptors mediates the cardiovascular effects of etomidate.' Anesthesiology 2003, 99, 4, 889-95
5. Brady, AE, et al. 'Spinophilin stabilizes cell surface expression of alpha 2B-adrenergic receptors.' J Biol Chem 2003, 278, 34, 32405-12
6. Savola, JM, et al. 'Fipamezole (JP-1730) is a potent alpha2 adrenergic receptor antagonist that reduces levodopa-induced dyskinesia in the MPTP-lesioned primate model of Parkinson's disease.' Mov Disord 2003, 8, 872-83
7. Sivenius, K, et al. 'A deletion in the alpha2B-adrenergic receptor gene and autonomic nervous function in central obesity.' Obes Res 2003, 8, 962-70
8. Ge, H, et al. 'Constitutive precoupling to G(i) and increased agonist potency in the alpha(2B)-adrenoceptor.' Biochem Biophys Res Commun 2003, 306, 4, 959-65
9. Sykiotis GP, et al. 'The alpha2B adrenergic receptor deletion/insertion polymorphism in morbid obesity.' Clin Auton Res 2003, 3, 203-7
10. Feldman J, Bousquet P. 'Role of cental alpha2B-adrenergic receptors in blood pressure control and hypertension.' J Hypertens 2003, 5, 871-2
11. Willems EW, et al. 'Possible role of alpha-adrenoceptor subtypes in acute migraine therapy.' Cephalalgia 2003, 4, 245-57.
UniProt: P19328.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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