Clone Number: 2G9A10F5
Immunogen: Human bone marrow malignant cells from a non- B, non-T acute leukemia
Concentration: n/a
Format: Purified
Storage buffer: Liquid purified Ascites, purified with Protein G Chromatography with 15mM Sodium Azide
Additional info: Originally, the 2G9 monoclonal antibody (mAb) was described as identifying a 15 kDa protein found in the cytoplasmic granules of cytotoxic T cells that might be part of a larger 40 kDa molecule, ubiquitously expressed, named p40-TIA-1 and often referred to as TIA-1 in the literature (1, 2). Now, however, there is evidence that the 2G9 mAb identifies a 17 kDa cytoplasmic granule membrane protein named GMP-17 that has no similarity with p40-TIA-1 (3). The GMP-17 antigen is a 165 amino acid protein with 4 transmembrane domains: but it is not a typical member of the fourtransmembrane superfamily. It is identical with previously identified cytotoxic granule proteins called NKG7 and GIG-1 – for GCSF induced gene protein 1 –, isolated from NK cells and granulocyte-colony-stimulatingfactor- treated mononuclear cells, respectively (4, 5). Pretreatment: Heat induced epitope retrieval in 10 mM citrate buffer, pH6.0, for 20 minutes is required for IHC staining on formalin-fixed, paraffin embedded tissue sections. Note: Dilution of the antibody in 10% normal goat serum followed by a goat anti-mouse secondary antibody-based detection is recommended. Control tissue Tonsil. Staining granular
References: Anderson, P. et al, 1990, J. Immunol., 2, 144, 574./Tian, Q., et al, 1991, Cell, 67, 629-639./Medley, et al, 1996, Proc. Natl. Acad. Sci. U S A, 93, 2, 685-9.
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