Clone Number: MAb11
Immunogen: Recombinant human TNF-alpha
Concentration: 1 mg/ml
Format: Purified by protein-A affinity chromatography.
Storage buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Additional info: TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.SpecificityThe mouse monoclonal antibody TPX2-01 recognizes the epitope EPFVPKKEKKS (aa 636-646 in human) of TPX2, a microtubule-associated intracellular critical regulator of mitosis, which is overexpressed in many types of tumors and is a marker of worse prognosis in various cancers.Application detailsELISA: Biotinylated MAb11 can be used as a detection antibody in combination with capture antibody MAb1.
Immunohistochemistry (frozen sections): paraformaldehyde-fixed, saponin-treated frozen tissue sections.
Flow cytometry: Intracellular staining.
English
Deutsch
