Products from BPS Bioscience require a minimum order value above 400€
Applications: Useful for cell culture and for the study of signaling pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.
Biological Activity: ED50 is 0.1 - 0.2 ng/ml as determined by a cell proliferation assay using BALB/c 3T3 cells.
Description: Mouse Epidermal Growth Factor (GenBank Accession No. NM_010113), a.a. 977- 1029, MW=6.0 kDa, expressed in an E. coli expression system.
Endotoxin Level: < 1EU per µg of EGF as determined by the LAL method.
Format: lyophilized protein
Formulation: Lyophilized from a sterile filtered solution containing 10 mM Phosphate Buffered Saline (PBS), pH 7.4, 138 mM NaCl, and 2.7 mM KCl.
Genbank: NM_010113
Purity: ≥98% by SDS-PAGE and HPLC analysis.
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: ≥2 years (lyophilized) at -20°C.
Tags: none
Uniprot: Q01279
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Michaylira, C.Z., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 291 (3), G472-G481
2. Duncan, E.A., et al. J. Biol. Chem. 281(18), 12521-12525 (2006)
English
Deutsch
