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Encompassing Amino Acids: 670-1210
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed in a Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 µM ATP. The reaction to place at 30°C for 40 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Description: Mouse EGFR (GenBank Accession No. NM_207655/NP_997538), a.a. 670-1210 with a C-terminal FLAG-tag, MW = 62 kDa, expressed in SF9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol
Genbank: NM_207655
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-tag
Uniprot: Q01279
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Mackenzie, J. et al., Mol Immunol. 2011 Jul,48(12-13):1578-85
2. Sadri, K. et al., Nucl Med Commun. 2011 Jul,32(7):563-9.