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Encompassing Amino Acids: 24-97
Amino Acid Sequence: HPGSIPT SCCFIMTSKK IPNTLLKSYK RITNNRCTLK AIVFKTRLGK EICADPKKKW VQDATKHLDQ KLQTPKP
Background: Eotaxin belongs to the platelet factor-4 family of chemokines. The gene symbol is SCYA11. The factor is being referred to also as Eotaxin-1 and has been renamed CCL11. Eotaxin induces substantial accumulation of eosinophils in the skin without significantly affecting the accumulation of neutrophils. Eotaxin is a potent stimulator of both guinea pig and human eosinophils in vitro. The Eotaxin receptor is a G-protein-coupled receptor selectively expressed in human eosinophils .
Biological Activity: Biological activity was determined by the ability to chemoattract human peripheral blood eosinophils using a concentration range of 0.1-20.0 ng/ml.
Description: Recombinant mouse Eotaxin is a disulfide-linked homodimeric protein consisting 75 amino acid residues, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Mouse Eotaxin mature chain was expressed in E. coli.
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Format: lyophilized protein
Formulation: Lyophilized from 0.2 µm filtered PBS solution, pH 7.4.
Genbank: P48298
Purity: ≥95% by SDS-PAGE and HPLC
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Uniprot: P48298
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Clin. Cancer Res., Apr 2009, 15: 2647 - 2656.
2. Int. Immunol., Feb 2009, 21: 187 - 201.
3. J. Immunol., Nov 2008, 181: 7390 - 7399.
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