Storage: 2-8℃/-20℃,12 months
Abbreviation: GLP-1
Usage: This ELISA kit applies to the in vitro quantitative determination of GLP-1 concentrations in serum, plasma and other biological fluids.
Tested Sample Types: Serum, plasma and other biological fluids
Type: Sandwich-ELISA
Assay Time: 3 h 30 min
Detection Type: Colormetric
Sensitivity: 5.67 pg/mL
Detection Range: 15.63-1000 pg/mL
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GLP-1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GLP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GLP-1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of GLP-1 . You can calculate the concentration of GLP-1 in the samples by comparing the OD of the samples to the standard curve.
Cross_reactivity: This kit recognizes Mouse GLP-1 in samples.No significant cross-reactivity or interference between Mouse GLP-1 and analogues was observed.
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