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Encompassing Amino Acids: 21-140
Amino Acid Sequence: HIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCRASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS
Background: Interleukin-4 is produced mainly by a subpopulation of activated T-cells (Th2) that are the biologically most active helper cells for B-cells and that also secrete IL-5 and IL-6. The biological activities of IL-4 are mediated by a specific receptor. The extracellular domain of the IL-4 receptor is related to the receptors for EPO, IL-6, and the beta chain of the IL2 receptor (CD124). Two types of IL-4 receptor (IL-4R) exist: the type 1 receptor is a heterodimer consisting of CD132 and IL-4R-alpha. The type 2 receptor is a heterodimer consisting of IL-4Ralpha and IL-13Ralpha1. IL-4 enhances expression of MHC class 2 antigens on B-cells. It can promote their capacity to respond to other B-cell stimuli and to present antigens for T-cells. Pretreatment of macrophages with IL-4 prevents the production of IL1, TNF-alpha and prostaglandins in response to activation of the cells by bacterial endotoxins or IFN-gamma. IL-4 synergises with EPO and G-CSF in the generation of colonies containing granulocytes or erythroid progenitor cells in a colony formation assay.
Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is ≤ 0.4 ng/ml, corresponding to a specific activity of≥ 2x 10^6 units/mg.
Description: Recombinant mouse Interleukin-4 is a disulfide-linked monomer protein consisting of 121 amino acid residues, migrates as an approximately 14 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding mouse Interleukin-4 mature chain was expressed in E. coli.
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Format: lyophilized protein
Formulation: Lyophilized from a 0.2 µm filtered PBS solution, pH 7.0.
Genbank: P07750
Purity: ≥96% by SDS-PAGE and HPLC
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Uniprot: P07750
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tachdjian R, et al. J. Exp. Med., Sep 2009, 206: 2191 - 2204.
2. Yu, M., et al. J Biol Chem. 2009 Nov 20,284(47):32968-79.
3. Ennaciri J, Girard D. J Immunol. 2009 Oct 15,183(8):5261-9
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