Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: 2-751
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: Histone demethylase that demethylates Lys-4 of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 Lys-9 or H3 Lys-27. Demethylates trimethylated, dimethylated and monomethylated H3 Lys-4. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma.
Description: Mouse JARID1B, also known as PLU-1 and KDM5B (GenBank Accession No. NM_152895), a.a. 2-751 with N-terminal FLAG-tag, MW = 86 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 ?g/ml FLAG peptide, 10% glycerol, and 3 mM DTT
Genbank: NM_152895
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: Q80Y84
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Yamane K, et al. (2007). Mol. Cell 25 (6): 801-12.
2. Barrett A, et al. (2007). Int. J.Cancer 121 (2): 265-75.Application Reference:
An high-throughput in vivo screening system to select H3K4-specific histone demethylase inhibitors (2014)
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