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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: JNK1 is a member of the MAP kinase group that is activated by dual phosphorylation at thr and tyr residues during exposure to stress such as UV irradiation. JNK1 binds to the c-Jun transactivation domain and phosphorylates it on Ser-63 and Ser-73 (1). JNK1 has been shown to play an important role in disease processes. Activation of JNK1 results in defects in myotube viability and integrity leading to dystrophic myofiber destruction (2). JNK1 activity is also abnormally elevated in obesity and removal of JNK1 results in decreased adiposity and significantly improved insulin sensitivity.
Description: Mouse JNK1 (GenBank Accession No. NM_016700), full length with N-terminal GST-tag, MW=71 kDa, expressed in a Baculovirus infected Sf9 cell expression system. The mouse amino acid sequence of JNK1 is identical to the one of human JNK1.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_016700
Host Cell Line: Sf9 cells
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of 0.5 mg/ml ATF2 substrate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Derijard, B. et al. Cell. 1994 Mar 25,76(6):1025-37.
2. Kolodziejczyk, S M. et al. Curr Biol. 2001 Aug 21,11(16):1278-82.
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