Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: MEK1 (EE) is a member of the dual specificity protein kinase family that acts as a mitogen-activated protein kinase (MAPK) kinase. MEK1 lies upstream of MAPK/ERK and stimulates the enzymatic activity of MAPK/ERK upon a wide variety of extra- and intracellular signals. As an essential component of MAPK/ERK signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development (1). Constitutive activation of MEK1 results in cellular transformation. Thus, MEK1 represents a likely target for pharmacologic intervention in proliferative disease such as cancer (2).
Description: Recombinant mouse MEK1 (S218E, S222E), full length with Serine to Glutamic acid mutations on a.a. 218 and 222 and an N-terminal GST-tag, expressed in E. coli cells.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_008927
Purity: ≥95%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Seger, R. et al: The MAPK signaling cascade. FASEB J. 9: 726-735, 1995.
2. Sebolt-Leopold, J S. et al: Blockade of the MAP kinase pathway suppresses growth of colon tumors in vivo. Nature Med. 5: 810-816, 1999.
English
Deutsch
