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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: PAK3 is a member of the family of serine/threonine p21-activating kinases that serve as targets for the small GTP binding proteins Cdc42 and RAC (1). The PAK family of proteins have been implicated in a wide range of biological activities and are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK3 seems to be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. A point mutation in PAK3 gene has been linked to nonsyndromic X-linked mental retardation (2).
Description: Recombinant mouse PAK3, full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_008778
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Manser, E. et al: Molecular cloning of a new member of the p21-Cdc42/Rac-activated kinase (PAK) family. J. Biol. Chem. 270: 25070-25078, 1995.
2. Bienvenu, T. et al: Missense mutation in PAK3, R67C, causes X-linked nonspecific mental retardation. Am. J. Med. Genet. 93: 294-298, 2000.
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