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Applications: This kit is perfect for screening for inhibitors of mouse PD-L1 binding to mouse PD-1.
Background: Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and is exploited by both tumors and viruses for immune evasion.
Description: The Mouse PD-1[Biotinylated]:PD-L1 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this signaling. The key to this kit is the high sensitivity of detection of biotin-labeled PD-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, mouse PD-L1 is coated on a 96-well plate. Next, mouse PD-1 is incubated with mouse PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
Storage Stability: One year from date of receipt when stored as directed.
Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled mouse PD-1, purified mouse PD-L1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Lin, D., et al. Proc. Natl. Acad. Sci. USA. 2008, 105: 3011-3016.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.
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