Mouse UCP-1 (Uncoupling Protein 1, Mitochondrial) CLIA Kit

Abbreviation: UCP-1

Target Synonym: SLC25A7;Thermogenin;

Target Species: Mouse

Usage: This ELISA kit applies to the in vitro quantitative determination of Mouse UCP-1 concentrations in Tissue homogenates.

Tested Sample Types: Tissue homogenates

Assay Time: 3.5h

Sensitivity: 18.75 pg/mL

Detection Range: 31.25-2000 pg/mL

UNIProt ID: P12242

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse UCP-1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse UCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse UCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse UCP-1. You can calculate the concentration of Mouse UCP-1 in the samples by comparing the RLU value of the samples to the standard curve.

Cross reactivity: This kit recognizes Mouse UCP-1 in samples.No significant cross-reactivity or interference between Mouse UCP-1 and analogues was observed

• SKU: ELSE-CL-M0452-48
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-E-CL-M0452-48
• Package Unit: 48 T
• Quantity Unit: PAK
• Application: Chemiluminescence Immunoassays (CLIA)