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Applications: Useful for cell culture and for the study of signaling and developmental pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.
Biological Activity: ED50 is less than 2 ng/ml as determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC).
Description: Recombinant mouse VEGF (GenBank Accession No. NM_009505) is a 39 kDa disulfide-linked homodimeric protein (a.a. 27-190), consisting of two 164 amino acid polypeptide.
Endotoxin Level: < 1EU per µg of VEGF as determined by the LAL method.
Format: lyophilized protein
Formulation: Lyophilized from 0.2 µm filtered 20 mM PBS solution, pH 7.0
Genbank: NM_009505
Purity: ≥98% by SDS-PAGE and HPLC analysis.
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: ≥2 years (lyophilized) at -20°C. Reconstituted solutions can be stored in undiluted aliquots at 4°C forone week or at -20°C for up to six months.
Tags: none
Uniprot: Q00731
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Lee, S., et al. Cell 130 (4), 691-703 (2007)
2. Yamamoto, H., et al. Dev. Biol. 308 (1), 44-53 (2007)
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