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Encompassing Amino Acids: 1-473
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: Myotonic Dystrophy Kinase-Related cdc42-binding kinase beta (MRCKβ) belongs to the DMPK subfamily (1). The myotonic dystrophy kinase-related kinases and myotonic dystrophy kinase-related Cdc42 binding kinase (MRCK) are effectors of RhoA and Cdc42, respectively, where they are involved in actin cytoskeletal reorganization and neurite outgrowth (2). Effects of the repeat expansion on the DMPK gene may be responsible for muscle and heart features of Myotonic Dystrophy.
Description: Recombinant human Myotonic Dystrophy Kinase-Related cdc42-binding kinase beta (MRCKβ), a.a. 1-473, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_006035
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9Y5S2
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Lam. L T. et al: Characterization of a monoclonal antibody panel shows that the myotonic dystrophy protein kinase, DMPK, is expressed almost exclusively in muscle and heart. Hum. Mol. Genet. 2000; 9(14): 2167-73.
2. Tan, I. et al: Phosphorylation of a novel myosin binding subunit of protein phosphatase 1 reveals a conserved mechanism in the regulation of actin cytoskeleton. J. Biol Chem. 2001; 276(24):21209-16.
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