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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: MST4 belongs to a family of proteins that share similarity with a budding yeast serine/threonine kinase, sterile-20 (Ste20), and have a role in mitogen-activated protein kinase signaling during cytoskeletal rearrangement, morphogenesis, apoptosis, and other diverse cellular events (1). Expression of MST4 has been shown to correlate with tumorigenicity and inversely with androgen receptor status in a number of human prostate cancer cell lines. Overexpression of MST4 induced anchorage-independent growth and increased in vitro proliferation of these prostate cancer cell lines (2).
Description: Recombinant human MST4, also known as MASK (GenBank Accession No. NM_016542), full length, with N-terminal GST tag, MW=72 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_016542
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9P289
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Dan, I. Et al: Cloning of MASK, a novel member of the mammalian germinal center kinase III subfamily, with apoptosis-inducing properties. J. Biol. Chem. 277: 5929-5939, 2002.
2. Sung, V. et al: The Ste20 kinase MST4 plays a role in prostate cancer progression. Cancer Res. 63: 3356-3363, 2003.