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Application: Screen therapeutic antibodies and ADCs targeting MUC1MUC1 vaccination with or without co-treatment targeting MUC1
Background: MUC1 (mucin-1) is a transmembrane protein involved in cell adhesion in epithelial cells as well as helping to provide a layer of protection against bacterial and enzyme attack. It is broadly expressed in human epithelial tissues however, MUC1 is also an oncogene that is overexpressed in ovarian, breast, gastric and some non-epithelial tumors. Aberrant expression of MUC1 upregulates cell growth, metastasis, proliferation and other functions in cancer and correlates with poor prognosis and survival time in patients. The extracellular region of MUC1 is highly glycosylated (O- and N-linked carbohydrates and sialic acid) and contains a polymorphic region of variable number of tandem repeats (VNTR) region as well as a SEA (sperm protein, enterokinase and agrin) domain. These glycosylated regions help form a physical barrier towards exogenous pathogens and participate in defense against pathogens. The SEA domain contains a cleavage site which has been indicated to also play a role in defense against microorganisms. This cell line expresses isoform 2 which is highly expressed in tumor tissues. While the cytoplasmic tail region and associated signaling functions from MUC1 isoform 1 are preserved, isoform 2 has a truncated extracellular domain that lacks the VNTR region and a 15 amino-acid deletion within the SEA domain. As a result, it does not undergo cleavage. Due to its cell surface expression in a variety of cancers and correlation with patient prognosis, MUC1 is a prospective therapeutic target for treatments including CAR-T Cell Therapy, MUC1-activated T cell treatment and MUC1 vaccination.
Description: MUC1 CHO Cell Line is a CHO-K1 cell line expressing MUC1 variant 2 (also known as Mucin-1 or CD227; NM_001018016.3) under the control of a cytomegalovirus (CMV) promoter. This cell line was generated by lipid-mediated transfection followed by geneticin selection and limited dilution. Individual clones were screened for MUC1 expression levels by flow cytometry, and a clone was selected to generate this cell line.
Host Cell Line: CHO-K1 cells, Chinese Hamster Ovary, epithelial-like cells, adherent
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.
Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
References: Nivet C., et al., 2024 Biomedicines 12(1), 139.Kumar S., et al., 2017 Carcinogenesis 38(7): 671-679.Chen W., et al. 2021 Int J Mol Sci. 22(12): 6567.
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