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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: MYLK2 is a member of the myosin light chain kinase family and is a calcium/calmodulin dependent enzyme that is exclusively expressed in adult skeletal muscle (1). MYLK2 has been proposed to participate in signaling pathways (calcium signaling pathway, focal adhesion, regulation of actin cytoskeleton) and cellular processes (neuromuscular synaptic transmission, protein/amino acid phosphorylation). MYLK2 is involved in multiple molecular functions as a result of various subdomains that participate in ATP binding, calmodulin binding, nucleotide binding, protein serine/threonine kinase activity and transferase activity) (2).
Description: Recombinant human MYLK2, full length, with N-terminal His-tag, expressed by Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM sodium phosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol
Genbank: NM_033118
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q9H1R3
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Soung, Y.H. et al:, Mutational analysis of the kinase domain of MYLK2 gene in common human cancers.Pathol Res Pract. 2006;202(3):137-40.
2. Toth-Zsamboki, E. et al. P2X1-mediated ERK2 activation amplifies the collagen-induced platelet secretion by enhancing myosin light chain kinase activation.J. Biol Chem. 2003; 21;278(47):46661-7.
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