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Encompassing Amino Acids: 2-491 (end)
Applications: Useful for the study of protein binding assays, enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The reaction mixture contained 50 mM Tris-HCl, pH 8.0, 12.5 mM MgCl2, 0.1 mM nicotinamide, 0.4 mM PRPP,2 mM ATP, 30 µg/ml of alcoholdehydrogenase, 10 µg/ml of NMNAT (BPS,#71090), 1.5% alcohol, 1 mM DTT, 0.02%BSA, and various amount of NAMPT in a finalvolume of 50 µl. Reactions were initiated withthe addition of NAMPT, and the increase inNADH fluorescence (Ex 340 nm/Em 460 nm)was continuously monitored for 30 min atroom temperature.
Description: Human Nicotinamide phosphoribosyltransferase protein (NAMPT), also known as PBEF1 or visfatin (GenBank Accession No. NM_005746) a.a. 2-491, with N-terminal GST-tag, MW=82 kDa, expressed in an E. coli cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 10 mM glutathione, 0.04% Tween-20, 20% glycerol.
Genbank: NM_005746
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P43490
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Amal B et al., Mol. Cell. Biol. 14 (2)
2. Martin PR. et al., J. Bacteriol. 183 (4)
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