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Encompassing Amino Acids: 2-491(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The 50 µl reaction mixture contained 50 mM Tris-HCl, pH 8.0, 12.5 mM MgCl2, 0.1 mM nicotinamide, 0.4 mM PRPP, 2 mM ATP, 30 µg/ml of alcohol dehydrogenase, 10 µg/ml of NMNAT (BPS, #71090), 1.5% alcohol, 1 mM DTT, 0.02% BSA. Reactions were incubated at room temperature for 30 minutes, and the increase in NADH fluorescence (Ex 340 nm/Em 460 nm) was measured.
Background: Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging.
Description: Human Nicotinamide phosphoribosyltransferase protein (NAMPT), also known as PBEF1 or visfatin (GenBank Accession No. NM_005746), a.a. 2-491 (end), no tag, MW=56 kDa, expressed in an E. coli cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol.
Genbank: NM_005746
Storage Stability: At least 6 months at -80°C.
Tags: none
Uniprot: P43490
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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