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Application: Isolate NCAM1/CD56 expressing NK cells from a mixed population such as PBMCs.Positively selected cell can be used in downstream applications such as genomic analysis, expression assays, protein isolation, and flow cytometry.
Background: Natural Killer (NK) cells are effector cells of the innate immune system. These lymphocytes display cytotoxic activity when activated and are of interest in the field of cancer immunotherapy. NCAM1/CD56 is a canonical marker of NK cells and is present in most stages of NK cell development. In PBMCs derived from healthy individuals, about 10-30% of the cells are NCAM1/CD56+ NK cells.
Description: The NCAM1/CD56 Positive Cell Isolation Kit is designed to magnetically separate NCAM1/CD56-expressing cells s from a complex immune cell population. This kit is optimized for the isolation of NCAM1/CD56-positive cells from normal human peripheral blood mononuclear cells (PBMCs). Your PBMCs are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. The NCAM1/CD56 positive cells are immobilized along the side of the tube by the magnet, while undesired NCAM1/CD56 negative cells remain in suspension for easy removal.
Storage Stability: This kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: This kit provides enough reagents and materials for isolation from up to 1 x 108 or 1 x 109 PBMCs. It is possible to use this kit for multiple isolations from smaller PBMC samples.
Uniprot: P13591
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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