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Encompassing Amino Acids: 1-302(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was performed in a 25 µl reaction containing 40 mM Tris, pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA, 100 µM ATP, 0.2 mg/ml Casein and NEK7 for 50 min at 30°C. NEK7 activity was measured using ADP-Glo reagents (Promega).
Description: Human NEK7, also known as NIMA Related Kinase 7 (GenBank Accession No. NM_133494), full length, a.a. 1-302 (end), with N-terminal GST-tag, MW=61.5 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT and 20% glycerol
Genbank: NM_133494
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q8TDX7
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kandli, M., et al. Genomics 68: 187-196 (2000).
2. Belham, C., et al. Curr. Biol. 15: 1155-1167 (2001).
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