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Encompassing Amino Acids: 1-979 (end), delta 346-731
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA and 1 mM DTT) containing 20 µM ATP and 0.2 mg/ml MBP for 30 minutes at 30°C. Enzyme activity is measured using Kinase-Glo plus reagents (Promega).
Description: Human NEK9, also known as NERCC (GenBank Accession No. NM_033116), a.a 1-345, 732-end (a.a. 1-979 with deleted fragment a.a. 346-731), with N-terminal GST-tag, MW =92 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 20% glycerol, and 3 mM DTT.
Genbank: NM_033116
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q8TD19
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Roig, J., et al. Genes Dev. 16: 1640-1658 (2002).
2. Belham, C., et al. Curr. Biol. 15: 1155-1167 (2001).
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