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Applications: Enzyme kinetics studies and screening small molecule inhibitors for drug discovery and high-throughput screening (HTS) applications.
Background: NEU2, also known as N-acetyl-alpha-neuroaminidase 2 or sialidase-2, belongs to the glycohydrolytic enzyme family. Glycoside hydrolases remove terminal sialic acid residues from sialo-glycoproteins and sialo-glycolipids. The human cytosolic sialidase NEU2 recognizes not only the a(2-3), a(2-8), and a(2-6) of the sialosyl linkage of the terminal sialic acid residue but also the inner sugar(s) of the oligosaccharide moiety, as well as the supramolecular organization of gangliosides. NEU2 has been implicated in several diseases such as cancer and type I and type II sialidosis. The PI3K-AKT pathway is upregulated in many cancers, with many of the proteins involved in this pathway being altered by sialylation, which can result in oncogene inactivation. Lower than average levels of NEU2 have been found in pancreatic cancer cells, resulting in decreased apoptosis. Inhibition of NEU2 is an important approach to decipher sialidase function and mechanism of action. The use of inhibitors may also prove useful as a therapeutic approach.
Description: The NEU2 Fluorogenic Assay Kit is designed to measure NEU2 (Neuroaminidase 2 or Sialidase-2) glycohydrolase activity for screening and profiling applications. It comes in a convenient 96-well format, with purified recombinant NEU2 enzyme, NEU2 substrate, NEU2 assay buffer, and Stop Solution for 100 enzyme reactions. The NEU2 inhibitor NCGC00063279 is also included as a control.Hydrolysation of the NEU substrate releases a fluorophore (λexc/λem = 365 nm/445 nm). The increase in fluorescence at λ=445 nm is directly proportional to NEU2 activity.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: Q9Y3R4
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: Khedri Z, et al., 2012, Org. Biomol. Chem 10: 6112-6120.
Mozzi A, et al., 2011, Proteins 80: 1123-1132.
Nath S., et al., 2018, Cell Death and Disease 9: 210.
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