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Applications: The NF-κB-luciferase/Jurkat cell line is suitable for monitoring the activity of NF-kB signaling in response to stimulant, and establishing cell-based screens for inhibitors that target specific NF-κB stimulating molecules.
Description: NF-κB luciferase reporter construct is stably integrated into the genome of Jurkat T- cells. The firefly luciferase gene is controlled by 4 copies of NF-kB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.
Format: Each vial contains ~2 x 10^6 cells in 1mL of 10% DMSO in FBS.
Host Cell Line: Jurkat
Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Cat. No. LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Cat. No. LT07-518) was used as a positive control.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
References: 1.Borner C and Kraus J (2013) Inhibition of NF-kB by Opioids in T cells. J Immunology. 191.2.Mori N. et.al. (2002) Bay 11-7082 inhibits transcription factor NF-kB and induces apoptosis of HTLV-1- infected T cell lines and primary adult T-cell leukemia cells. Blood. 100: 1828-18343.Takada Y, Kobayashi Y, Aggarawal BB (2005) Evodiamine abolishes constitutive and inducible NF-kappaB activation by inhibiting IkappaBalpha kinase activation, thereby suppressing NF-kappaB-regulated antiapoptotic and metastatic gene expression, up-regulating apopotiss and inhibiting invasion. J Biol Chem. 280: 17203
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