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Applications: Monitor NF-κB-signaling pathway activity Screen inhibitors of NF-κB
Description: The NF-κB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-κB) signal transduction pathways. The pack contains the NF-κB Reporter (Luc)- HCT-116 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-κB inhibitor, evodiamine.Additionally, the pack includes cell culture medium (Thaw Medium 7) that has been optimized for use with HCT-116 cells. Thaw Medium 7 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required.
Host Cell Line: HCT116
Storage Stability: Immediately upon receipt, store vials in liquid nitrogen.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Samuel T et.al. (2014) Variable NF-κB pathway responses in colon cancer cells treated with chemotherapeutic drugs. BMC Cancer 14: 599.2. Arabi A et.al. (2012) Proteomic screen reveals Fbw7 as a modulator of the NF-κB pathway. Nature Communication 3: 976.3. Clemo NK et.al. (2008) BAG-1 is up-regulated in colorectal tumour progression and promotes colorectal tumour cell survival through increased NF-κB activity. Carcinogenesis 29: 849.4. Tukhvatulin AI et.al. (2011) An In Vitro and In Vivo Study of the ability of NOD1 Ligands to Activate the Transcriptional Factor NF-κB. Acta Naturae 3: 77.
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