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Description: NF-B luciferase reporter construct is stably integrated into the genome of HCT-116 cells. The
firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of
the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors
bind to the DNA response elements to induce transcription of the luciferase gene.The NF-κB-luciferase/HCT-116 cell line is suitable for monitoring the activity of NF-κB signaling
in response to stimulants such as the cytokines TNF and IL-1β, pathogen-associated
molecular pattern (PAMP) (i.e. flagellin) or endogenous damage-associated molecular pattern
(DAMP) molecules (i.e. NOD1 ligand) (see application references). It is also suitable for
establishing cell-based screens for inhibitors that target specific NF-κB stimulating molecules.
This cell line can be further modified to allow investigation of downstream NF-κB activities as a
result of targeted genetic mutation(s).
Format: Aqueous solution containing DMSO
Host Cell Line: HCT116
Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Cat. #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, Cat. #LT07-518) was used as a positive control.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Biosafety Level: BSL-2
References: 1. Samuel T et.al. (2014) Variable NF-B pathway responses in colon cancer cells treated with chemotherapeutic drugs. BMC Cancer 14: 599.
2. Arabi A et.al. (2012) Proteomic screen reveals Fbw7 as a modulator of the NF-B pathway. Nature Communication
3: 976. 3. Clemo NK et.al. (2008) BAG-1 is up-regulated in colorectal tumour progression and promotes colorectal tumour cell survival through increased NF-B activity. Carcinogenesis 29: 849.
4. Tukhvatulin AI et.al. (2011) An In Vitro and In Vivo Study of the ability of NOD1 Ligands to Activate the Transcriptional Factor NF-B. Acta Naturae 3: 77.
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