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Application: Monitor NF-kB activity.Screen for activators or inhibitors of the NF-kB signaling pathway.Study the effect of compound on NF-kB activity and cell viability in parallel.
Background: Nuclear factor-Kappa B (NF-κB)/Rel proteins include NF-κB2 p52/p100, NF-κB1 p50/p105, c-Rel, RelA/p65, and RelB. These proteins function as dimeric transcription factors that control genes regulating a broad range of biological processes including innate and adaptive immunity, inflammation, stress responses, B cell development, and lymphoid organogenesis. It is ubiquitously present in almost all mammalian cells, and responds to cellular stress signals, such as stress, free radicals, UV radiation and bacteria and virus. Activation of NF-κB through tumor necrosis factor receptors (TNFR) occurs upon engagement with its respective ligand TNFα (tumor necrosis factor α). Activation of NF-κB enhances cell inflammation and prevents apoptosis, which contribute to tumor development. An understanding of the NF-κB pathway and how to modulate is critical to understand gene regulation in health and disease.
Description: NF-κB TWO-Luciferase Reporter HEK293 Cell Line is a HEK293 cell line designed to monitor cell viability alongside NF-κB (nuclear factor Kappa B) activity. It contains a firefly luciferase reporter driven by four copies of the NF-κB response element located upstream of the minimal TATA promoter. In addition, this cell line constitutively expresses Renilla Luciferase under the control of a CMV promoter, which can be used to determine cell viability.This cell has been validated with TNFα (tumor necrosis factor alpha), and the inhibitor IKK-16 dihydrochloride.
Host Cell Line: HEK293, Human Embryonic Kidney, epithelial-like cells, adherent.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.
Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: Chen W., et.al., 2011 Front. Biosci. 16: 1172-1185Schmeck B., et.al., 2007 Eur. Respir. J. 29: 25-33
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