Application Note: Anti-NF-Y (A subunit) Antibody was tested by immunoblot and found to be reactive against NF-Y (A subunit specific) at a dilution of 1:6,000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat). Anti-NF-Y (A subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y. Negligible reactivity was observed against the B subunit of NF-Y. Anti-NF-Y (A subunit) Antibody was also tested in a gel supershift assay and found to be reactive against NF-Y using 1.0 to 2.0 µl per assay. Anti-NF-Y was tested in IHC using human prostate tissue with 10µg/mL. Specific conditions should be optimized by user.
Concentration Value: 90 mg/ml
ELISA Dilution: 1:20,000 - 1:100,000
Gel Shift Dilution: Use 2.0 µL
Immunohistochemistry Dilution: 1:400
Western Blot Dilution: 1:2,000 - 1:10,000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: NF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Physical State: Liquid (sterile filtered)
Purity and Specificity: Anti-NF-Y (A subunit) Antibody was prepared from monospecific antiserum by delipidation and defibrination.
Background: Nuclear transcription factor Y subunit alpha is a protein that in humans is encoded by the NFYA gene. The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. Anti-NF-Y is ideal for investigators involved in transcription factor research.
Low Endotoxin: No
Other: User Optimized
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