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Application: Luciferase- based analysis of live and dead target cells in cytotoxicity assays.Test the efficacy of multi-specific immune engager molecules.Assess the Fc effector function of candidate antibodies.
Background: Lymphocyte-mediated cytotoxicity is a form of cellular immunity against intracellular pathogens, including viruses, certain bacteria, and parasites. The most commonly used in vitro methods to monitor lymphocyte-mediated cytotoxicity on target cells are cell-mediated cytotoxicity assays such as ADCC (Antibody-Dependent Cellular Cytotoxicity) and TDCC (T-cell Dependent Cellular Cytotoxicity) in which immune effector cells and target cells are co-cultured. To analyze immune effector cell cytolytic activity within this heterogeneous cell population of effector and target cells, it is important to discriminate between effector and target cell populations with distinct phenotypes. The use of luciferase allows for a clear separation between effector and surviving target cells. The instability of firefly luciferase when released from dead target cells in cell culture gives it a half-life of approximately 2 hours, eliminating any residual luminescence signal generated from dead target cells. Cytotoxicity assays are crucial for understanding the potency of CAR (Chimeric Antigen Receptor) T cells, NK cells, and antibody-based immunotherapies.NALM6 is a human B cell precursor leukemia cell line derived from the peripheral blood of a patient with acute lymphoblastic leukemia. The Firefly Luciferase NALM6 Cell Line constitutively expresses the Firefly (Photinus pyralis) luciferase reporter under the control of a CMV promoter. Luciferase activity is directly proportional to live cell numbers. These cells grow in suspension.
Description: The NK Cell Cytotoxicity Luciferase Assay Kit (NALM6) is designed to determine the cytotoxicity profile of NK (Natural Killer) cells towards the Firefly Luciferase NALM6 Cell Line. It uses the luminescence signal from Firefly Luciferase NALM6 Cell Line to measure the number of live target cells within a mixed cell population of NK and Firefly Luciferase NALM6 cells. The kit contains NK Cells, Firefly Luciferase NALM6 Cell Line, cell culture media and One-Step™ Luciferase Assay System.
Host Cell Line: NALM6
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-1
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