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Encompassing Amino Acids: 2-279
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The 50 µL reaction mixture contained 50 mM Tris-HCl, pH 7.4, 5 mM MgCl2, 0.1 mM NMN, 0.1 mM ATP, and various amounts of NMNAT. The reaction was incubated at 37°C for 30 min, and the amount of ATP consumed was calculated using Kinase-Glo® Plus reagent (Promega).
Description: Human nicotinamide nucleotide adenylyltransferase 1, also known as NMNAT1, (GenBank Accession No. NM_022787), a.a. 2-279 with N-terminal His-tag, MW = 32.8 kDa, expressed in an E. coli cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 256 mM imidazole and 20% glycerol
Genbank: NM_022787
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q9HAN9
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kaplan, J., et al., Med Sci (Paris). 2013; 29(1):26-27
2. Chiang, P.W., et al., Nat Genet. 2012; 44(9):972-974
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