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Encompassing Amino Acids: 1-264 (full length)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 0.05% Tween 20, 1 mM DTT, 100 µM Quinoline, 100 µM SAM, 30 min, room temperature.
Description: Human NNMT1 also known as Nicotinamide N-Methyltransferase, GenBank Accession No. NM_006169, a.a. 1-264(full length), with C-terminal His-tag, expressed in an E. coli expression system. MW=30 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3 mM DTT, 145 mM Imidazole.
Genbank: NM_006169
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: C-terminal His-Tag
Uniprot: P40261
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tan, H.H. and Porter, A.G. Biochem. Biophys. Res. Commun. 382 (2): 462-467 (2009).
2. Fan, H. et al., World J. Gastroenterol. 15 (16): 2020-2026 (2009).