Application Note: WB: 0.5 - 1.0 μg/mL. ELISA: 1:10000 - 1:40000. IHC: 2.5 μg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 130
Specificity/Sensitivity: At least five isoforms of Nogo are known to exist; this antibody is specific for Nogo A and Nogo E.
Form: Liquid
Buffer (with preservative): 0.02M Potassium Phosphate, 0.15M NaCl, 0.01% Sodium azide.
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Background: NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS; blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients.
Uniprot ID: Q9NQC3
Antigen Species: Human
Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Nogo A protein.
Purification: Purified by antigen-affinity chromatography.
Conjugation: Unconjugated
Full Name: reticulon 4
English
Deutsch
