Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The NSD2 Direct Activity Chemiluminescent Assay Kit is designed to measure NSD2activity for screening and profiling applications. The key to the NSD2 Chemiluminescent Activity AssayKit is a highly specific antibody that recognizes methylated residue of Histone H3. With thiskit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer andmethyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treatedwith an HRP-labeled secondary antibody followed by addition of the HRP substrate toproduce chemiluminescence that can then be measured using a chemiluminescencereader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Kit comes in a convenient format, with 96-well plate precoated with histone H3 substrate, reagents, and purified NSD2 enzyme for 96 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Dillon S.C., et al. 2005. Genome Biology 6:227.
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