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Encompassing Amino Acids: 1021-1322
Applications: Suitable for protein binding studies, as an immunogen, or as a Western blotting control.
Background: NSD3 is a histone methyltransferase that preferentially methylates Lys4 and Lys27 of histone H3. Lys4 methylation usually represents a specific tag for epigenetic transcriptional activation, while Lys27 is a mark for transcriptional repression.
Description: Human Nuclear SET domain-containing protein 3 (NSD3), also known as WHSC1L1, GenBank Accession No. NM_023034, a.a. 1021-1322, with N-terminal GST-tag, MW=61 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT and 20% glycerol.
Genbank: NM_023034
Host Cell Line: E. coli
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9BZ95
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Morishita, M., di Luccio E., Biochim Biophys Acta. 2011 Dec,1816(2):158-63.
2. Rahman, S., et al., Mol Cell Biol. 2011 Jul,31(13):2641-52.
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