Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and HTS applications.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The NSD3 Chemiluminescent Assay Kit is designed to measure NSD3 activity for screening and profiling applications. The key to the NSD3 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, Sadenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Storage Stability: Stable at least one year from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: The NSD3 Chemiluminescent Assay Kit comes in a convenient format, with 384-well plate precoated with a specific substrate, the antibody against methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransfer
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Dillon, S.C., et al. Genome Biology 2005; 6:227.Bennet, R.L., et al. Cold Spring Harb Perspect Med. 2017; 7(6): a026708.
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