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Applications: Positive control in NY-ESO-1 TCR-T development.Screen modulators of NY-ESO-1 TCT-T driven cytotoxicity.Design and optimize co-culture bioassays for NY-ESO-1-specific TCR cell evaluation.
Background: NY-ESO-1 (New York esophageal squamous cell carcinoma 1), also known as Cancer/testis antigen 1 or CTAG1B, is an important tumorigenic marker present in malignant cells. Normally expressed only in embryonic testis, this highly immunogenic protein is not usually found in normal tissues, but is re-expressed in multiple myeloma, non-small cell lung carcinoma (NSCLC), and breast and ovarian cancer, making it a promising candidate antigen for cancer immunotherapy. Several NY-ESO-1-directed therapies are being developed including cancer vaccines, anti-NY-ESO-1 adoptive cell therapy, and NY-ESO-1-specific TCR-T cell therapy in combination with checkpoint inhibitors.
Clone Number: c259
Description: The NY-ESO-1 (c259) TCR-T Cells are generated by high-titer lentiviral transduction of human primary CD4+ and CD8+ T cells with NY-ESO-1-Specific TCR Lentivirus (Clone c259) (#78676). These ready-to-use TCR (T cell receptor) -T cells express the human TCR clone c259, that specifically recognizes the antigen NY-ESO-1 (New York esophageal squamous cell carcinoma 1).These TCR-T cells have been validated by flow cytometry (to determine the TCR expression) and co-culture assays (IFN-γ staining and degranulation).
Mycoplasma Testing: The cells have been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.
Supplied As: Each vial contains 5 x 106 cells in 1 ml of CryoStor® CS10 (Stemcell Technologies #100-1061)
Warnings: Donors have been screened and determined negative for:Hepatitis B (anti-HBc EIA, HBsAg EIA)Hepatitis C (anti-HCV EIA)Human Immunodeficiency Virus (HIV-1/HIV-2 plus O)Human T-Lymphotropic Virus (HTLV-I/II)HIV-1/HCV/HBVWest Nile VirusTrypanasoma cruziNote: Testing cannot guarantee that any sample is completely virus-free. These cells should be treated as potentially infectious and appropriate biological safety level 2 (BSL-2) precautions should be used.
Biosafety Level: BSL-1
References: Thomas R., et al., 2018 Front. Immunol. 9: 00947.
Raza A., et al., 2020 J. of Translational Medicine 140.
Kropp KN., et al., 2020 PLOS One 15(9): e0238875.
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