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Applications: Useful for studying protein binding, screening small molecules, and in vitro cellular assays.
Description: Human secreted OX40, also known asCD134, Tumor Necrosis Factor ReceptorSuperfamily Member 4, and TNFRSF4,GenBank Accession No. NM_003327, a.a.29-216 with C-terminal Avitag™ and Histag,expressed in a HEK293 cell expressionsystem. This protein is enzymatically biotinlabeledusing Avitag™ technology. MW =23.6 kDa (monomer). This protein runs at ahigher MW by SDS-PAGE due toglycosylation. Biotinylation was confirmed tobe >90%.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Genbank: NM_003327
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal Avi-tag™ and His-tag
Uniprot: P43489
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Rogers, P.R. et al., Immunity. 2001; 15(3): 445-455.
2. Ito, T. et al., J. Exp. Med. 2005; 202(9): 1213-1223.