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Applications: Useful for the study of RAS GTPase activation.
Assay Conditions: GTPase activity of KRAS(WT, 50ng) and KRAS(G12C, 100ng) were tested in the presence of various concentration of p120GAP (0 ng - 200 ng) and 2 µM GTP by using GTPase-Glo reagents (Promega). Reactions were done at room temperature for 80 min.
Description: Human Ras GTPase-activating protein 1, also known as p120GAP, RASA1, GAP and RASA, GenBank Accession No. NM_002890, a.a. 714-1047(end) with an N-terminal His-Tag, expressed in an E. coli cell expression system. MW=39 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol.
Genbank: NM_002890
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal His-Tag
Uniprot: P20936
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Scheffzek, K., et al., Nature. 1996; 384: 591-596
2. Torit, M. et al., JBC. 1992; 267: 8293-8298
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