P3 Prim. Cell 4D-Nucleofector™ LV Kit XL

P3 Primary Cell 4D-Nucleofector LV Kit XL

The P3 Primary Cell 4D-Nucleofector™ LV Kit XL* is suited for larger scale transfection of primary cells in the LV Nucleocuvette™ Cartridge format when working with the 4D-Nucleofector™ LV Unit. With the LV Nucleocuvette™ Cartridge a variable volume of up to 20 mL can be transfected. The cartridge is automatically filled and emptied with help of the supplied input and output reservoirs. Alternatively bags can be used. An optional second input allows for separate feeding of mRNA to avoid its degradation.
*Note: From the five primary cell solutions (P1 – P5) that come with the 4D-Nucleofector™ System only P3 is available for the large-scale formats so far. Solution P3 is suited for most immune and stem cells (e.g. T cells, dendritic cells, CD34 hematopoietic stem cells).

Content:
•22.5 mL P3 Primary Cell Nucleofector™ Solution
•5 mL Supplement 1,
•50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
•1 x 1 mL Nucleocuvette™ Cartridge
•2 x 4D-Nucleofector™ LV Reservoir

• SKU: LONV4LP-3020
• Manufacturer: Lonza
• Manufacturer SKU: V4LP-3020
• Package Unit: ca. 10 million cells PEL
• Quantity Unit: PAK
• Type: Nucleofection Kit