Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 1-663(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction was performed in a buffer(50 mM Tris, pH 7.4, 50 mM NaCl, 10mM CaCl2, and 2 mM DTT) containingPADI1 and 10 mM BAEE as a substratefor 25 min at 37°C. Amount of ammoniaproduced was measured and used forspecific activity calculation.
Description: Human PADI-1, also known as Peptidyl arginine deiminase, type I, (GenBank Accession No. NM_013358), 1-663(end), with C-terminal FLAG-tag, MW= 75 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 3 mM DTT and 10% glycerol.
Genbank: NM_013358
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-tag
Uniprot: Q9ULC6
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kanno T, et al. (2000). J. Invest. Dermatol. 115 (5): 813-23.
2. Nachat R, et al. (2005). J. Invest. Dermatol. 124 (2): 384-93.
3. Guerrin M, et al. (2003). Biochem. J. 370 (Pt 1): 167-74.
English
Deutsch
